Table of properties of different domains that bind to phospho-peptides.

(The binding is at phospho-serine / phospho-threonine / phospho-tyrosine denoted by X)

(Look out for the above color code)

No

 

Binding Domain

Domain

Str. Eg.

Examples: Structure - Protein - Binding site - View

Consensus binding site* Prosite Entry

Annotation - References

1

FHA

1DMZ, 1FHQ,

1G3G,

1J4O,

1K3J,

1QU5.

1G6G

No name.

SLEVXEADATFAK.

EDIXYLD.

In eukaryotes, many FHA domain-containing proteins localize to the nucleus, where they participate in establishing or maintaining cell cycle checkpoints, DNA repair, or transcriptional regulation. [1], [2], [3], [4].

1FHR

DNA repair protein.

EDIXYLD.

1GXC

Synthetic.

RHFDXYLIRR.

1J4K

DNA repair protein.

EDIXYLD.

1J4L

DNA repair protein.

EVELXQELP.

1J4P

DNA repair protein.

KKMTFQXPTDPLE.

1J4Q

DNA repair protein.

SLEVXEADATFVQ.

1K2M

DNA repair protein.

EDIXYLD.

1K2N

DNA repair protein.

EVELXQELP.

1K3N

DNA repair protein.

KKMTFQXPTDPLE.

1K3Q

DNA repair protein.

SLEVXEADATFVQ.

2

14_3_3

1A38, 1O9C, 1O9E.

1A37

Ps-Raf259 Peptide.

KSQRQRSTXTPNVHM.

QSYXV.

14-3-3 homologues mediate signal transduction by binding to phosphoserine containing proteins. [5], [6].

1QJA

Phosphopeptide.

RLYHXLPA.

1QJB

Phosphopeptide.

ARSHXYPA/RSHXYPA

1O9D

Plasma Membrane H+ Atpase.

QSYXV.

1O9F

Plasma Membrane H+ Atpase.

QSYXV.

3

WW

1EOM, 1I6C, 1PIN,

1J6Y.

1F8A

Y(Sep)Pt(Sep)S Peptide.

YXPTXPS.

YXPTXPS.

Domain with 2 conserved Trp (W) residues; Also known as the WWP or rsp5 domain. Binds proline-rich polypeptides. [7], [8], [9].

1I8G

M-Phase Inducer Phosphatase 3.

EQPLXPVTDL.

1I8H

Microtubule-Associated Protein Tau.

KVSVVRXPPKSPS.

4

Vhs

1ELK, 1DVP, 1JWF, 1MHQ.

1LF8

Ci Man-6-P Receptor.

FHDDXDEDLLHI.

FHDDXDEDLLHI.

Domain present in VPS-27, Hrs and STAM. [10], [11].

5

HprK

1JB1, 1KKL.

1KKM

Phosphocarrier Protein Hpr.

~TVNLKXIMGVM~

~TVNLKXIMGVM~

Serine kinase of the HPr protein regulates carbohydrate metabolism [Signal transduction mechanisms]. [12], [13].

6

DSP

1VHR.

1J4X

DDE(Ahp)(Tpo)G

(Ptr)VATR.

DDEXXGXVATR.

DDEXXGXVATR.

Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Characterized as VHR- or Cdc25-like. [14].

7

KIX

1JJS.

1KDX

Creb.

~SRRPXYRKIL~

~SRRPXYRKIL~

CBP and P300 bind to the CREB via a domain known as KIX. The KIX domain of CBP also binds to transactivation domains of other nuclear factors including Myb and Jun. [15].

8

MH2

1DD1.

1KHX

Smad2 Protein.

~VRCSXMX.

~VRCSXMX.

MH2 domain. This is the MH2 (MAD homology 2) domain found at the carboxy terminus of MAD related proteins. The MH2 domain mediates interaction with a wide variety of proteins. [16], [17].

9

WD40

1ERJ.

1NEX

GLL(Tpo)PPQSG.

GLLXPPQSG.

GLLXPPQSG.

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. [18].

10

PKI

 

1STC

Capk. SEP & TPO.

~AAKKGXEQESV~ ~EEIRVXINEKC~ ~KGRTWXLCGTP~

~EEIRVXINEKC~ ~AAKKGXEQESV~

~KGRTWXLCGTP~

cAMP and cGMP

cAMP-dependent protein kinase inhibitor (PKI). Members of this family are extremely potent competitive inhibitors of camp-dependent protein kinase (Capk) activity. These proteins interact with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains. [23], [19], [20], [21], [22], [24].

1FMO

Capk. SEP & TPO.

~EEIRVXINEKC~ ~KGRTWXLCGTP~

1YDR

Capk. SEP & TPO.

~EEIRVXINEKC~ ~KGRTWXLCGTP~

1YDS

Capk. SEP & TPO.

~EEIRVXINEKC~ ~KGRTWXLCGTP~

1YDT

Capk. SEP & TPO.

~EEIRVXINEKC~ ~KGRTWXLCGTP~

1JBP

Capk. SEP & TPO.

~AAKKGXEQESV~ ~EEIRVXINEKC~ ~KGRTWXLCGTP~

1JLU

Capk. SEP & TPO.

(Domain is also phosphorylated)

~EEIRVXINEKC~ ~KGRTWXLCGTP~

(~RTGRRAXIHD.)

1L3R

Capk. SEP & TPO.

~AAKKGXEQESV~

~RIGRFXEPHAR~

~EEIRVXINEKC~ ~KGRTWXLCGTP~

11

API3

1F32.

1F34

Pepsin A.

~TFEATXQELSI~

~TFEATXQELSI~

Ascaris pepsin inhibitor-3 (API3); protein inhibitor that reversibly inhibits aspartic proteinase cathepsin E, and gastric enzymes pepsin and gastricsin. [25].

12

ARM

1G3J, 1IAL.

1I7W

Epithelial-Cadherin.

~VFDYEGXGXEAASL-XSLNSS~

~VFDYEGXGXEAASLXSL-NSS~

ARM has been implicated in mediating protein-protein interactions, but no common features among the target proteins recognized by the ARM repeats have been identified. [26], [27], [28], [29].

13

Cyclin

1VIN.

1E9H

Cdk2.

~PVRTYXHEVVT~

~PVRTYXHEVVT~

Protein binding domain functioning in cell-cycle and transcription control. Present in cyclins, TFIIB and Retinoblastoma (RB). The cyclins consist of 8 classes of cell cycle regulators that regulate cyclin dependent kinases (CDKs). [38], [30], [31], [32], [33], [34], [35].

1GY3

Cdk2. P33 protein kinase.

~PVRTYXHEVVT~

1H1P

Cdk2. P33 protein kinase.

~PVRTYXHEVVT~

1H1Q

Cdk2. P33 protein kinase.

~PVRTYXHEVVT~

1H1R

Cdk2. P33 protein kinase.

~PVRTYXHEVVT~

1H1S

Cdk2. P33 protein kinase.

~PVRTYXHEVVT~

1H24

Cdk2. P33 protein kinase.

~PVRTYXHEVVT~

1H25

Cdk2. P33 protein kinase.

~PVRTYXHEVVT~

1H26

Cdk2. P33 protein kinase.

~PVRTYXHEVVT~

1H27

Cdk2. P33 protein kinase.

~PVRTYXHEVVT~

1H28

Cdk2. P33 protein kinase.

~PVRTYXHEVVT~

1QMZ

Cdk2. P33 protein kinase.

~PVRTYXHEVVT~

1JSU

Cdk2.

~PVRTYXHEVVT~

14

CDI

 

1FQ1

Cell Division Protein Kinase 2.

~PVRTYXHEVVT~

~PVRTYXHEVVT~

Cyclin-dependent kinase inhibitor. Cell cycle progression is negatively controlled by cyclin-dependent kinases inhibitors (CDIs). CDIs are involved in cell cycle arrest at the G1 phase. [36].

15

GlgA

1H8F,

1I09.

1O6L

Serine/Threonine Protein Kinase

~GATMKXFCGTP~

~GATMKXFCGTP~

Glycogen synthase [Carbohydrate transport and metabolism]. [37].

1O6K

Serine/Threonine Protein Kinase

~GATMKXFCGTP~

16

SH2

 

 

 

???

SH2

SH2 domains typically bind pTyr-containing ligands via two surface pockets, a pTyr and hydrophobic binding pocket, allowing proteins with SH2 domains to localize to tyrosine phosphorylated sites.

17

StKc

 

 

 

???

 

 

18

Psa

 

1BJN

 

???

 

[1].

19

Psp

 

1F5S

 

???

 

[1].

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

??? Interesting domains for which structures are not readily available.

* - Consensus from binding sites listed in column 4 and from data in Prosite (if available).