CDC2-RPA Interactions (literature search)

Summary References Sequence Structure

Summary:

RPA residues	CDC2 binding site	Reference
RPA34 (Ser-23 and Ser-29 get phosphorylated)	Specific site yet to be found^§	Hurwitz (Sep. ’93) Dixon (Sep. ’97)
RPA70 (weak interaction)	Specific site yet to be found^§	Hurwitz (Sep. ’93)

^§ - Exact domains of interaction are yet to be determined.

References:

A. Information on specific interaction sites

1. Dixon et al. – Sep. 1997.

Ser-23 and Ser-29 are confirmed to be the residues that get phosphorylated

by cdc2 protein kinase. (TOP)

2. Hurwitz et al. – May 1997.
3. Hurwitz at al. – Sep. 1993.

RPA34 was found to be phosphorylated by cdc2 protein kinase with

cyclin B1 or cyclin A.

RPA70 was also phosphorylated by cdc2 with cyclin B but to a lesser extent.

4. Stillman et al. – June 1992. (TOP)

B. General interaction information

1. Dixon et al. – Mar. 2003.
2. Lee et al. – Jan. 2002.
3. Melendy et al. – Jan 2000.
4. Wold et al. – Sep. 1994.
5. Stillman et al. – 1992. (TOP)

C. CDC2 Full Sequence

Source: NP_001777 – CDC2 on NCBI protein sequence database [gi: 4502709].

1 MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR EISLLKELRH

61 PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQYM DSSLVKSYLY QILQGIVFCH

121 SRRVLHRDLK PQNLLIDDKG TIKLADFGLA RAFGIPIRVY THEVVTLWYR SPEVLLGSAR

181 YSTPVDIWSI GTIFAELATK KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNT

241 FPKWKPGSLA SHVKNLDENG LDLLSKMLIY DPAKRISGKM ALNHPYFNDL DNQIKKM

D. RAD52 Structure (TOP)

The tertiary structure of RAD52 is yet to be determined. Its secondary structure predicted by SABLE server : CDC2.

The tertiary structure of RPA32 N-terminal is also yet to be determined.