p53-RPA Interactions (literature search)

Summary     References     Sequence     Structure

 

Summary:

RPA residues

p53 binding site

Specific Reference

RPA70N (1-221)

and

RPA70C (411-492)

p53N (1-73)

critical domain: p53 (40-60)

Dutta (July ’96)

Li (July ’97)

 

References:

 

A. Information on specific Interaction sites

 

  1. Dutta et al. – Jun. 1996.

N-terminal domain of p53 interacts with RPA.                                       (TOP)

 

  1. Dutta et al. – July 1996.

RPA70N (1-221 aa) interacts with p53. RPA70C (411-492) interacts with p53.

Above RPA70 domains are potential binding sites for other proteins.

Zinc-finger motif in RPA70 is not needed for binding to p53, RPA34 or ssDNA.

p53 binds RPA70 by excluding ssDNA from the complex.                                (TOP)

 

  1. Prives et al. – Apr. 1997.

“p53 does not inhibit ssDNA binding by RPA”.

“RPA is unable to complex with p53 in the presence of ssDNAs”.

RPA interacts with p53’s N-terminus and possibly C-terminus.              (TOP)

 

  1. Li et al. – July 1997.

First 73 aa of p53 is sufficient for interaction with RPA.

Residues 40-60 are the most critical for binding with RPA.

UV radiation inhibits RPA-p53 interaction.                                                        (TOP)

 

  1. Lowry et al. – Aug. 1999.

RPA70N interacts with p53.                                                                             (TOP)

 

B. General interaction information

 

  1. Prosperi et al. – Dec. 2001.
  2. Melendy et al. – Mar. 1998.                                                                             (TOP)

 

C. Other papers

 

  1. Dutta et al. – Sep. 1993.
  2. Grosse et al. – Jan. 1999.
  3. Pommier et al. – Mar. 1999. – Info on DNA-PK.                    
  4. Bohr et al. – June 2000.
  5. Pommier et al. – 2000.                                                                         (TOP)

 

D. p53 Full Sequence

 

Source: AAD28628 – p53 on NCBI protein sequence database [gi: 4732147].

 

  1 MEEPQSDPSV EPPLSQETFS DLWKLLPENN VLSPLPSQAM DDLMLSPDDI EQWFTEDPGP

 61 DEAPRMPEAA PRVAPAPAAP TPAAPAPAPS WPLSSSVPSQ KTYQGSYGFR LGFLHSGTAK

121 SVTCTYSPAL NKMFCQLAKT CPVQLWVDST PPPGTRVRAM AIYKQSQHMT EVVRRCPHHE

181 RCSDSDGLAP PQHLIRVEGN LRVEYLDDRN TFRHSVVVPY EPPEVGSDCT TIHYNYMCNS

241 SCMGGMNRRP ILTIITLEDS SGNLLGRNSF EVRVCACPGR DRRTEEENLR KKGEPHHELP

301 PGSTKRALPN NTSSSPQPKK KPLDGEYFTL QIRGRERFEM FRELNEALEL KDAQAGKEPG

361 GSRAHSSHLK SKKGQSTSRH KKLMFKTEGP DSD

 

E. p53 Structure

 

Many structures are available for p53 in PDB. In fact they have a special page dedicated for this. Click on either of the links below:

1.      p53 @ PDB.

2.      p53 entries @ PDB.

 

The structure of the RPA70 domains which interact with p53 (1-221, 411-492) are yet to be determined.